Part:BBa_K1994025:Design

BsaI-GFP-dCas9

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 40
  Illegal NheI site found at 2067
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4346
  Illegal XhoI site found at 70
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 106
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 14
  Illegal BsaI.rc site found at 2
  

Design Notes

GFP-dCas9

with bsa1 cut sites for Golden Gate assembly upstream of the GFP gene

Source

S. Pyrogenes